Determination of ultratrace zinc by enzymatic activity of carbonic anhydrase. II. Use of carbonate hydro-lyase activity.
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概要
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A method for the determination of trace zinc has been investigated using hydro-lyase activity of carbonic anhydrase. It was proved theoretically and experimentally that the activation rate of the apo-carbonic anhydrase with zinc is proportional to the amount of zinc present in the solution. Hydro-lyase activity was measured electrochemically using sodium hydrogencarbonate as a substrate. The limit of detection was 0.89 ng/ml or 40 pg of zinc, lower than that obtained by the esterase activity method. Application of the present method to the determination of zinc in real samples has been examined in order to evaluate its analytical feasibility.
- 公益社団法人 日本化学会の論文
著者
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Kobayashi Kensei
Department Of Chemistry And Biotechnology Faculty Of Engineering Yokohama National University
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Fujiwara Kitao
Department Of Chemistry Faculty Of Science University Of Tokyo
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Haraguchi Hiroki
Department of Agricultural Chemistry, Faculty of Agriculture The University of Tokyo
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Fuwa Keiichiro
Department of Chemistry and Physics, National Institute for Environmental Studies
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Fuwa Keiichiro
Department of Agricultural Chemistry, Faculty of Agriculture The University of Tokyo
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