Determination of ultratrace zinc by enzymatic activity of carbonic anhydrase.
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概要
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A method for the determination of trace zinc has been investigated by using enzymatic activity of carbonic anhydrase. The recovery of enzymatic activity of apo-carbonic anhydrase was proportional to the amount of zinc in solution theoretically and experimentally, when it was monitored spectroscopically using the substrate, <I>p</I>-nitrophenyl acetate. The amount of zinc in the sample solutions was therefore determined from the curve, zinc concentration <I>vs</I>. the enzymatic activity. The limit of detection is 10 ng/ml or 2 ng. The method has been applied to the analysis of zinc in fruit juices and water samples.
- 公益社団法人 日本化学会の論文
著者
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Kobayashi Kensei
Department Of Chemistry And Biotechnology Faculty Of Engineering Yokohama National University
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Fujiwara Kitao
Department Of Chemistry Faculty Of Science University Of Tokyo
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Haraguchi Hiroki
Department of Agricultural Chemistry, Faculty of Agriculture The University of Tokyo
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Kobayashi Kensei
Department of Chemistry, Faculty of Science, The University of Tokyo
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Fuwa Keiichiro
Department of Agricultural Chemistry, Faculty of Agriculture The University of Tokyo
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