Enzyme sensors based on coated-wire electrode. Use of carboxyl-substituted poly(vinyl chloride) as a support for immobilizing penicillinase.

概要

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A poly(vinyl chloride) derivative which contained 1.8% carboxyl residue was used to prepare a potentiometric sensor sensitive to penicillin G. A pH-sensitive base electrode was fabricated by covering an Ag wire electrode with the membrane composed of the carboxyl-substituted poly(vinyl chloride), <I>o</I>-nitrophenyl octyl ether, and tridodecylamine. The sensitive layer of the pH-sensitive electrode was modified with penicillinase to make a penicillin-sensitive electrode. Penicillinase was adsorbed to the surface of the polymer membrane by immersing the sensitive layer of the electrode in the penicillinase solution. Treatment of the pH-sensitive electrode with ca. 0.3% penicillinase solution at pH 6.7 (1 mM phosphate buffer) resulted in favorable performance of the penicillin sensor. The long-term stability of the penicillin sensor thus prepared was satisfactorily good.
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