Radioimmunoassayによる血漿11-deoxycorticosterone (DOC) 含量の測定法

スポンサーリンク

概要

• 論文の詳細を見る

• A radioimmunoassay procedure has been developed to measure 11-deoxycorticosterone (DOC) in human peripheral plasma. DOC-oxime was coupled with porcine gamma globulin and antibodies produced in rabbits. One to 3 ml of plasma, with 1, 2 <SUP>3</SUP>H-DOC added for recovery, was extracted with dichloromethane and purification achieved by a silica gel column and by one paper chromatograph. After overnight incubation of the antibodysteroid mixture at 4°C, bound and free fractions were separated using ammonium sulfate. The mean recovery of <SUP>3</SUP>H-DOC, after extraction and chromatography, was 84.6 ± 7.4%. The method showed adequate specificity, precision and accuracy. <BR>Normal plasma DOC levels were found to be 4.4 ± 2.5 ng/100 ml (n=8). Plasma DOC levels were almost normal (0.3-26.8 ng/100 ml) infifteen patients with benign essential hypertension. The mean level of 8.1 ± 8.2 ng/100 ml obtained in hypertensive patients with suppressed plasma renin activity, was not significantly different from normal. Plasma DOC showed a high level, 3.0-30.5 (11.4 ± 7.5) ng/100 ml, in 9 patients with primary aldosteronism. Four out of 8 patients with Cushing's syndrome were found to have elevated plasm a DOC levels. Higher levels of 21.2 ± 15.8 ng/100 ml were found in 5 patients with adrenal hyperplasia than those of 12.3 ± 8.0 ng/100 ml in 3 with adrenal adenoma. Plasma DOC levels were high levels of 113-176 ng/100 ml in 2 patients with 17α-hydroxylase deficiency. ACTH administered to 5 subjects produced a mean increase in plasma DOC from 4.8 to 25.8 ng/100 ml. Angiotensin II infused at a rate of 10 ng/kg/min for 30 min into 4subjects did not increase mean plasma DOC. Similarly, dietary sodium restriction or postural change did not increase plasma DOC. <BR>These results confirm that DOC secretion is primarily under anterior pituitary control. From the basal level of 4.4 ng/100 ml and from its biological activity compared to aldosterone, the major mineralocorticoid, it would seem that DOC plays a minor role in electrolyte homeostasis in normal humans. However, the elevated levels seen in some cases of adrenal cortical hyperfunction may mean that DOC contributes significantly to the characteristic symptoms of hypertension and hypokalemia.

• 日本内分泌学会の論文

著者

• 福地 総逸

  東北大学医学部第2内科学教室

• 中嶋 凱夫

  東北大学医学部第二内科

• 竹内 孝彦

  東北大学医学部第2内科

• 西里 弘二

  東北大学医学部第2内科

• 福地 総逸

  東北大学医学部第2内科

• 中嶋 凱夫

  東北大学医学部第2内科

関連論文

• 腎動脈瘤8症例の検討
• 高血圧症とアルドステロン
• 高血圧症とアルドステロン
• 高血圧症とアルドステロン
• 副腎スキャン剤131I-19-コレステロールの生体内代謝
• 原発性アルドステロン症における血漿アルドステロン含量について:I.レニン・アンギオテンシン系とアルドステロン
• 腎静脈また副腎静脈血中アルドステロン含量測定による原発性アルドステロン症の副腎腺腫の局在の診断
• Radioimmunoassayによる血漿11-deoxycorticosterone (DOC) 含量の測定法
• Radioimmunoassayによるアルドステロン分泌量の研究
• ヒト副腎皮質腫瘍に於けるACTHreceptorについて
• 甲状腺機能充進症におけるレニン・アンギオテンシン・アルドステロン系についての研究
• 原発性アルドステロン症の血漿アルドステロン含量:II.アルドステロンとACTH

もっと見る 閉じる

スポンサーリンク