TSHラジオイムノアッセイにおける高感度測定法の検討ならびにその臨床的意義

概要

論文の詳細を見る
Using a commercially available h-TSH RIA kit (Daiichi), a sensitive RIA method was developed.<BR>When pooled serum from patients with active Graves' disease was used as a carrier, combined applications of a delayed addition of 125-1 TSH and utilization of ×2 diluted anti-TSH antibody resulted in the consistent detection of 0.156μU/ml level of serum TSH. Further, 0.078μU/ml level could also be detected when 200μl of serum samples were applied. However, usage of 200μl sample volume should be determined after further studies concerning possible non specific effects of human serum.<BR>Assay results of 29 patient sera by the sensitive assay correlated quite well (r=0.953, Y=0.97X-0.06) with those by the regular assay. Serum TSH in all of the 20 normal subjects were detectable and ranged from 0.31 to 3.2μU/ml giving mean values of 1.48 ± 0.65 (s.d.) μU/ml. Values less than 0.156μU/ml were observed in patients with untreated Graves' disease, acute stage of subacute thyroiditis, and panhypopituitarism, and in postoperative cancer patients under suppressive doses of T-4.<BR>In conclusion, this sensitive TSH RIA made clear discrimination of low and normal serum TSH possible, and the clinical application of this method was considered quite useful.
日本内分泌学会の論文