Monoamine Oxidaseに関する研究-61-ラット肝Monoamine Oxidaseの電顕細胞化学的検出法

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The ultrastructural demonstration of monoamine oxidase (MAO) activity in rat liver was studied by the ferricyanide method. The modification of the ferricyanide method by Hanker et al. were as follows : the fixing time in situ with 2% paraformaldehyde (PFA) was 10 minutes, the volume of dimethylsulfoxide was 1/4 of the method of Hanker et al. and the fixed tissues in incubation medium were immersed at 25°C for 18 hours.<BR>The effect of perfused PFA on MAO activity in rat liver mitochondria was studied. When 1, 2, 4, 8 and 10% PFA solution were perf used, MAO activities with tyramine as substrate were 98, 90, 70, 55 and 43% of the control values, respectively. The substrate specificities of MAO in rat liver mitochondria fixed with 2% PFA were shown as the same pattern as the control rat, nevertheless these activities were lower than those of the control. By means of this modified method, the ultrastructural demonstration of MAO activity in rat liver cells was studied. The strong MAO activity was observed as electron dense area, both in the inner and the outer mitochondrial membrane. However, the main localization of MAO on which inner or outer membrane was not recognized. While, slight MAO activities were also observed in the other ultrastructural parts, such as mitochondrial cristae, endoplasmic reticulum and nuclear membrane. When rat liver was immersed in the incubation medium without tryptamine or pretreated with 10<SUP>-3</SUP> M pargyline, MAO activity could not detected. On the other hand, at 6 hours after administration of pargyline 25 mg/kg i.p. a slight MAO activity was observed in mitochondrial membrane, but at 12 hours after the administration no MAO activity was observed.

Monoamine Oxidaseに関する研究-61-ラット肝Monoamine Oxidaseの電顕細胞化学的検出法

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