ポリエチレングリコ-ルによる魚類精子の融合〔英文〕

スポンサーリンク

概要

• 論文の詳細を見る

• In chromosome manipulations of fish, duplication of genomes has been achieved generally by inhibiting the nuclear division. Recently, it has been also reported that genomes can be duplicated through fertilization with fused spermatozoa. In this study, fusion conditions of polythylene glycol (PEG) and motilities of fused spermatozoa were examined by using rainbow trout and nigorobuna spermatozoa. A comparison between two molecular weights of PEG indicated that a molecular weight of 4000 was superior in fusion ability to that of 1000. Fusion rate [(total number of spermatozoa participating in fusion/total number of tested spermatozoa) × 100] of rainbow trout spermatozoa was higher than that of nigorobuna. In a molecular weight of 4000, fusion rates of rainbow trout spermatozoa at concentrations of 30, 40, and 50% were 28.4, 40.2, and 44.4% respectively, while those of nigorobuna were 5.0, 18.6, and 34.8 respectively. When the diameter of the micropyle is taken into consideration, fused spermatozoa capable of entering an egg are restricted to those consisting of two spermatozoa. The maximum fusion rates between two spermatozoa in rainbow trout and nigorobuna were respectively 18.50% (concentration: 40%) and 15.9% (concentration: 50%). The duration of the motility of PEG-treated spermatozoa did not vary significantly depending on the three treatment concentrations, and compared with the control ones, the motion times of those of rainbow trout and nigorobuna were shortened by 2/3 and 1/2, respectively.

• 日本水産學會の論文

著者

• 上野 紘一

  近畿大学農学部水産増殖学研究室

• 井戸本 純一

  滋賀県水産試験場

• 上野 紘一

  近畿大学農学部

関連論文

• 日本産タナゴ亜科魚類の核型と系統的類縁との関連
• 三倍体アマゴの安静時および強制運動後の酸素消費量ならびに鰓蓋運動数
• RAPD分析による琵琶湖産フナ属魚類の種・亜種判別およびヨシ帯に出現するフナ仔稚魚の季節変化
• 多精受精をめざしたプロタミンによるフナ精子の接着
• 日本産キンギョの由来および品種保存の現状
• 雌性発生アマゴ卵の第二極体放出阻止最適時期に関する細胞学的検証
• ポリエチレングリコ-ルによる魚類精子の融合〔英文〕
• シマドジョウ属にみられた染色体多型と倍数性,ならびにそれらの染色体型の地理的分布
• 日本産モロコ類の核学的分類

スポンサーリンク