遊離アルギニンの酵素分解を併用した筋中ホスホアルギニンの定量法〔英文〕
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概要
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An attempt was made to increase the precision of estimation for phosphoarginine which was known as one of the phosphagens. To avoid the contraction of muscle as stimulated by the cold, the sample was frozen in liquified propane instead of liquid nitrogen, homogenized and extracted with perchloric acid solution. Phosphoarginine in the extract was determined by the increase in free arginine on acid-hydrolysis. But the increase was often little compared with the amount before hydrolysis, and the precision of estimation reduced greatly. This shortcoming was overcome by removing endogenous free arginine by arginase. When phosphoarginine was hydrolyzed and estimated by the Sakaguchi reaction, the coefficient of variation was about 4%.
- 日本水産學會の論文
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