培地のpHがEtham-butolの結核菌最低発育阻止濃度に及ぼす影響
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The effect of pH of media on the minimal inhibitory concentration of D-ethambutol to. Mycobacterium tuberculosis in vitro was investigated. Media used in this study were 1%Ogawa's egg slant and Kirchner's semi-liquid agar media with 10% albumin.<BR>pH of the condense water of Ogawa's medium was adjusted to 6.0, 6.4, 6.8, 7.2 and 7. if by adding suitable amount of 4% H<SUB>2</SUB>SO<SUB>4</SUB> and 10% Na<SUB>2</SUB>CO<SUB>3</SUB> to basal media of 1% Ogawa's egg slant. D-ethambutol was added to each of them at the concentration of 0, 1, 2.5, 5 and 10mcg/m<I>l</I>. The solution was mixed sufficiently, and 5 ml of them were dispensed into test tubes.<BR>pH of basal medium of Kirchner's semi-liquid agar media was adjusted to 6.0, 6.4, 6.8, 7.2 and 7.6 by adding suitable amount of 4% H<SUB>2</SUB>SO<SUB>4</SUB> and 10% Na<SUB>2</SUB>CO<SUB>3</SUB>. After cooling down, albumin was added at the concentration of 10%. D-ethambutol was added to each of them at the concentration of 0, 1, 2.5, 5 and 10 mcg/m<I>l</I>. They were mixed sufficiently, and 5 ml of them were dispensed into test tubes. Before their use, they were stored in an incubator at 37°C for 24 hours so as to confirm the sterility.<BR>The following 7 strains were used i n this study: H<SUB>37</SUB>Rv, A oyama-B, 3 strains isolated from previously untreated pulmonary tuberculosis patients and 2 strains isolated from patients who. had been treated by anti-tuberculosis chemotherapeutics including D-ethambutol.<BR>The results obtained were summarized as follows:<BR>1) The bacteriostatic effect of D-ethambutol was s tronger in alkaline than in acid on both the media.<BR>2) Inter-individual variation of the minimal inhibitory concentration of D-ethambutol to'susceptible strains of tubercle bacilli was the least in the media with pH 6.8 interpreted at 3'weeks after inoculation on both the media. The minimal inhibitory concentration of D-ethambutol under this condition was 2.5 mcg/m<I>l</I> on 1% Ogawa's slant and it was 5 mcg/m<I>l</I> in Kirchner's semi-liquid agar media.
- 一般社団法人 日本結核病学会の論文
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