1%小川培地とAlbumin加kirchner半流動培地における接種菌量ならびに培養期間がα-ethyl-thioisonicotinamide(1314TH)の結核菌最低発育阻止濃度に及ぼす影響

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Studies were made about the effect of the amount of bacilli for inoculation and the period of incubation on the minimal inhibitory concentration of 1314 TH to the growth of Myc. tuberculosis. One % Ogawa's media and Kirchner's semi-liquid agar media with albumin in 10%, were used. The concentrations of 1314 TH of the media were so prepared as to become 0, 1, 5, 10, 20, 50, 100 and 200 mcg/ml respectively. These media were mixed thoroughly, dispensed into test tubes in 5 ml quantity, and used after being stored in an incubator at 37°C for 24 hours in order to confirm the sterility. In consideration of our previous experience, the inoculations were made immediately after the preparation of the media. Three strains were used for this experiment, that is, H37Rv, Aoyama-B and a strain isolated from the sputum of a pulmonary tuberculosis patient without any history of previous treatment. These strains were planted on 1% Ogawa's egg media, and 2 week old cultures were always used for the experiment. Bacillary suspensions were prepared from these cultures, and by using electric photometer the amounts for inoculation were so prepared as to become 10-1, 10-2, 10-3 and 10-4 mg in 0.1 ml. After the inoculation, the media were incubated at 37°C fo r 6weeks and submitted to the weekly observation of the growth of colonies. The following results were obtained. The minimal inhibitory conc e ntration of 1314 TH to the growth of Myc. tuberculosis tends to become higher with the increasing amount of inoculum. In either medium the effect of incubation peri o d on the minimal inhibitory concentration was very marked, the latter increasing obviously with prolonged incubation period. Such effect was more obvious in 1% Ogawa's medium than in Kirchner's medium. When in Ogawa's medium 10-3 mg of inoculum was employe d, the minimal inhibitory concentrations of 1314 TH were 5 mcg/ml, 20 mcg/ml and 50 mcg/ml respectively in 2 week-, 3 weekand 4-5 week-old cultures. The corresponding values in Kirchner's medium were 5 mcg/m/and 10 mcg/ml in 2 week- and 3 week-old cultures. The marked rise in the minimal inhibito r y concentration observed in the case of prolonged incubation period is probably due to the decrease of drug potency by the effect of a high temperature of the incubator.
一般社団法人日本結核病学会の論文

1%小川培地とAlbumin加kirchner半流動培地における接種菌量ならびに培養期間がα-ethyl-thioisonicotinamide(1314TH)の結核菌最低発育阻止濃度に及ぼす影響

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