各種mycoplasmaに及ぼすtrypsin,nagarseの影響

概要

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For the purpose of investigating the susceptibility of Mycoplasma organism to proteolytic enzymes, such as trypsin and nagarse, the Mycoplasma OK strain which had been reported elsewhere was examined first of all for stability against various kinds of suspending solutions. It was found that the organisms were stable in a phoshate buffered saline (pH 7.4) at 37°C for 3 hours. Therefore, the effective doses of trypsin and nagarse against the OK strain were estimated with this solution and under the conditions mentioned above. As a result, the viable organisms (approximately 1010 living cells) were found to decrease remarkably in number with a dose of 250 HUM/ml of trypsin or 200P UN/ml of nagarse.Similarly, 6 and 2 kinds of Mycoplasma organisms of human and rodent, respectively, were investigated for susceptibility to proteolytic enzymes principally under the same conditions. As a result, M. pneumoniae, M. hominis type I, and the OK strain were proved to be susceptible to both enzymes, but M. hominis type II, M. salivarium, and M. orale were not susceptible to either enzyme. M. pharyngis was resistant to trypsin, while M. pulmonis was susceptible to nagarse. It was concluded that the Mycoplasma organisms tested could be divided into two groups on the basis of susceptibility to proteolytic enzymes.
日本細菌学会の論文