平板培地最下層(Bottom Layer)内培養法によるブドウ球菌集落の観察

概要

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Staphylococcus aureus was studied in the bottom layer cultivation in the nutrient agar medium by using 9cm-petri dishes. Colony forming efficiencies were more efficient in the mixed or bottom layer cultivation systems than that of the surface.In the bottom layer cultivation, a sheet of colonies, with opaque center and homogeneous large round periphery, were characteristically formed at 18 hours after the cultivation. This type of colonies, other than those of opaque round, or spindle appearence of the deep colonies, had the similarities with those of L form of the organism which were called fried egg appearence. But the strains derived from this study were not identified as staphylococcal L form because of the reversion to the mother strain at the primary cultivation.Staphylococcal phages were effective to the colonies of that type. The destructive, or eaten effect by the phages to the colonies in the bottom layer cultivation were seen as like as the cytopathic effect of the tissue culture.Hemolytic staphyloccal strains were generally not hemolytic in the deep cultivation. But, in the surface cultivation, crystals were seen restrictively in the surface colonies of many of the hemolytic strains.
日本細菌学会の論文