炭疽菌培養液中の酸沈澱物質の抗原性に関する研究 : IV. 酸沈澱物質の一分画と菌体ならびに莢膜成分との抗原関係

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A fraction (F-3) which precipitated in higher titer with anti-B. anthracis serum was separated from the acid-precipitating substance which had been produced in culture fluids of B. anthracis and some exper-iments were perfurmed on the fraction for the purpose of elucidating whether it was identical with the capsular polypeptide or one of the degradation products of the cellular substances. Fraction F-3 was a substance which differed from the capsular polypeptide not only in amino acid composition but also in serological activity. The paper chromatographical analysis showed the presence of six or seven amino acids and two unidentified ninhydrin-positive components in the hydrolyzate of fraction F-3 and the presence of glutamic acid and a trace of alanine in the hydrolyzate of the capsular polypeptide. Fraction F-3 precipitated in high titer when anti-B. anthracis serum was used, but the capsular polypeptide gave no precipitate with the same antiserum.<BR>Fraction F-3 was serologically closely related to the acid-precipitating substance isolated from the crude extract of living cells of B. anthracis.
日本細菌学会の論文