Action of Vitamin E as Antioxidant in Phospholipid Liposomal Membranes as Studied by Spin Label Technique

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概要

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• The action of α-tocopherol in the phosphatidylcholine liposomal membranes was studied by spin label technique in order to elucidate the role of vitamin E in the membranes. The fluidity of the liposomal membranes decreased with increasing concentrations of satu-rated phosphatidylcholine, cholesterol, and α-tocopherol. However, the physical effect of α-tocopherol was quite small at its physiological concentrations. The effects of structure of various kinds of chromanols on the membrane fluidity were studied. The fluidity of the liposomal mem-branes decreased with increasing length of the side chain at 2-position of 6-chromanols. This effect was smaller when the side chain at the 2-position did not possess branched methyl group. The effect of tocopherols on the membrane fluidity was larger in the inner part of the membranes. Ascorbic acid residing in the aqueous phase interacted with the nitroxide spin probe incorporated into the membranes and the rate was smaller as the nitroxide group was buried deeper in the phospholipid bilayer. The spin probes incorporated into liposomal membranes were consumed as the oxidation proceeded. α-Tocopherol suppressed both the oxidation of membranes and the decrease of spin probe, but when α-tocopherol was depleted, the spin probe decreased and the membranes became more rigid as the oxidation proceeded.

著者

• 浦野 四郎

  Tokyo Metopolitan Institute of Geronotology

• 二木 鋭雄

  Department of Reaction Chemistry, aculty of Engineering, University of Tokyo

• 高橋 希之

  Department of Reaction Chemistry, Faculty of Engineering, University of Tokyo

• 土屋 順一

  Department of Reaction Chemistry, Faculty of Engineering, University of Tokyo

関連論文

• Biosynthesis of Griseofulvin : Further ^C and ^2H NMR Studies
• The Synthesis of C-13 Labeled Vitamin E, [12'a, 13'-^C] all-rac-α-Tocopherol
• Synthesis of dl-α-Tocopherol and dl-α-Tocotrienol
• ^C Nuclear Magnetic Resonance Studies on α-Tocopherol and Its Derivatives
• Action of Vitamin E as Antioxidant in Phospholipid Liposomal Membranes as Studied by Spin Label Technique
• Free Radical Chain Oxidation and Hemolysis of Erythrocytes by Molecular Oxygen and Their Inhibition by Vitamin E
• Reactions of Some Tocopherols with the Methyl Radical
• Interaction of vitamin E and its model compounds with unsaturated fatty acids in homogeneous solution.
• Membrane stabilizing effect of vitamin E: Existence of a hydrogen bond between .ALPHA.-tocopherol and phospholipids in bilayer liposomes.
• Antioxidant activity of ubiquinol in solution and phosphatidylcholine liposome.
• Free radical-mediated damage of blood and its inhibition by antioxidants.

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