Effects of the Yeast Extract Components Pyrroloquinoline Quinone and Aspartic Acid on Vitamin B12 Production in Klebsiella pneumoniae IFO 13541

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概要

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• The production of vitamin B12 from carbohydrates, peptone, casamino acid, etc., by intestinal bacteria was investigated. Klebsiella pneumoniae IFO 13541 was the most efficient strain for vitamin Laboratory of Food Microbiology, Department of Food Science, Faculty of Home Economics, Mukogawa Womens University production, which depended exclusively on the concentration of yeast extract added to the medium. A concentrated solution of yeast extract (1 ml) was chromatographed on a Sephadex G-25 column (1×180 cm) and eluted with H2O (eighty fractions of 3 ml each were collected). It was found that fractions in which bacterial growth was most prevalent also exhibited the highest amount of vitamin production. The effectiveness of yeast extract was shown by the participation of pyrroloquinoline quinone and aspartic acid in the growth stimulation and in the vitamin B 12 production in this strain.

著者

• 中尾 祥子

  Laboratory of Food Microbiology, Department of Food Science, Faculty of Home Economics, Mukogawa Women's University

• 大杉 匡弘

  Laboratory of Applied Microbiology, Department of Food Science, Faculty of Home Economics, Mukogawa Women's University

• 近藤 由美

  Laboratory of Food Microbiology, Department of Food Science, Faculty of Home Economics, Mukogawa Women's University

• 野田 裕子

  Laboratory of Food Microbiology, Department of Food Science, Faculty of Home Economics, Mukogawa Women's University

• 室 佳予子

  Laboratory of Food Microbiology, Department of Food Science, Faculty of Home Economics, Mukogawa Women's University

• 石場 敦子

  Laboratory of Food Microbiology, Department of Food Science, Faculty of Home Economics, Mukogawa Women's University

• 室 佳予子

  Laboratory of Food Microbiology, Department of Food Science, Faculty of Home Economics, Mukogawa Women's University

• 石場 敦子

  Laboratory of Food Microbiology, Department of Food Science, Faculty of Home Economics, Mukogawa Women's University

関連論文

• Effects of the Yeast Extract Components Pyrroloquinoline Quinone and Aspartic Acid on Vitamin B12 Production in Klebsiella pneumoniae IFO 13541
• Microbial formation of biocytin.
• Biosynthesis of biotin-vitamers from unsaturated higher fatty acids by bacteria.
• Microbiological activity of biotin-vitamers.
• Biosynthesis of biotin-vitamers by family enterobacteriaceae.
• Formation of a biotin precursor, pimelic acid, in yeasts from C18 fatty acids.

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