生体親和性セラミック-骨形成因子複合体の骨形成能について第1報-ハイドロキシアパタイト-骨形成因子複合体

概要

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Bone Morphogenetic Protein (BMP) was extracted from bovine corticalbone and was bound to hydroxyapatite (HAP), which was synthesized by the wet method inorder to produce fine primary particles. Precipitate of the synthesized HAP was dried at 80°C, fired at 800°C for 3 hours, pulverized then sintered at 1200°C for 8 hours with the addition of resin beads, gclatin and hydrogenperoxide to make porous powder HAP and block HAP. For the combination of BMP and HAP, two different kinds of methods were performed.(Procedure 1) BMP was suspended in distilled water, and HAP was immersed in it, then lyophilized.(Procedure 2) BMP was solubilized in 0.01 M phosphate buffer (pH 7.2) with 6 M urea, and HAP was immersed in it, then dialyzed and lyophilized. HAP-BMP complexes were implanted in the thigh muscle pouch of mice (conventional male ddy, 5 weeks) which were sacrificed 3 weeks later to investigate the osteoinductive activity and biocornpatibility of the implants. All implanted HAP-BMP complex showed better osteoinductive activity than the implantation of BMP only, especially in the group made by prccedure 2, all implants induced fairly large amounts of new bone. No inflammatory cells were observed, and HAP and induced new bone were attached directly together without any connective tissues.
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