Du Français au Japonais par le truchement du Hollandais. Difficultés rencontrées par nos premiers traducteurs: A propos de la Nouvelle Méthode des Langues Françoise et Hollandoise par Pieter Marin(Amsterdam, 1775)

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The purpose of this study was to clarify the mechanism responsible for the different sensitivity of cells to fluoride (F) using four normal human diploid fibroblasts derived from the fetal lung and adult gingiva. The following results were obtained: 1. The activity of cell growth in decreasing order was NDU-1 cells (fetal lung) >GF-12-Y cells [adult gingiva, population doubling level (PDL): 10-15] >FR 30 cells (F-resistant cells produced from NDU-1 cells) >GF-12-E cells (PDL; 30-35). 2. The doubling time of cells in decreasing order was GF-12-E>FR 30>GF-12-Y>NDU-1 cells. 3. In nonsynchronized cultures, the F sensitivity of cells estimated by measuring the F concentration necessary to inhibit the DNA synthesis and/or the growth of cells in decreasing order was NDU-1>GF-12-Y>GF-12-E, FR 30 cells. 4. The time of S phase and pre-DNA phase was measured using synchronized cultures. The time of S phase was about 7 to 8 hrs in all four cell types. The time of pre-DNA phase was about 10 hrs in NDU-1 cells, about 20 hrs in GF-12-Y cells, about 32 hrs in FR 30 cells, and about 34 hrs in GF-12-E cells. 5. The DNA synthesis of synchronized cells at the S phase was depressed to about 50% of control cells for all four cell types when cultured with 0.79mM F for 8 hrs (time of S phase). A lethal F concentration was not observed in the cells in the pre-DNA phase. These results indicate that the F sensitivity of cells was dependent on the phase of the cell cycle. The different sensitivities to F that have been observed between cells (nonsynchronized) is probably releated to the fact the cells were in different phases of the cell cycle.
早稲田大学大学院文学研究科の論文

Du Français au Japonais par le truchement du Hollandais. Difficultés rencontrées par nos premiers traducteurs: A propos de la Nouvelle Méthode des Langues Françoise et Hollandoise par Pieter Marin(Amsterdam, 1775)

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