卵白フラボプロテインの簡易精製法

概要

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A simple procedure of the preparation of hen egg white flavoprotein was reported, in which the flavoprotein was adsorbed by DEAE-Sephadex A-25 batch-wise directly from egg white, and eluted in a column with 0.05M acetate buffer (pH 4.0) containing 0.5M NaCl after washing the Sephadex with the same buffer (pH 5.5) containing 0.1 and 0.15 M NaCl. At this step, the flavoprotein was refined up to 95% in purity on acrylamide gel electrophoresis. Further purification of the flavoprotein was conducted by gel filtration of Sephadex G-100 and pure flavoprotein on acrylamide disk electrophoresis was obtained. The apo-protein was prepared by treatment with charcoal in acidic pH and by dialysis, without loss of riboflavin binding property.
社団法人日本農芸化学会の論文