ペプチド抗生物質コリスチン生産菌, Bacillus colistinusの溶菌バクテリアによるペプチド抗生物質生産の生理 (第2報)

概要

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Intact cells of Bacillus colistinus which are resistant to lysozyme could be made more sensitive to the enzyme by the pretreatment with trypsin. After trypsin pretreatment cells could be converted into spheroplasts by incubation with lysozyme and ethylenedi-aminetetraacetic acid (EDTA) in the presence of sucrose and magnesium ion as the osmotic stabilizer in atmosphere of nitrogen, but those obtained in atmosphere of air were unstable. Spheroplasting of trypsin-treated cells by lysozyme without EDTA resulted in relatively much residue of bacilli and magnesium ion was effective on stabilization of spheroplasts induced by lysozyme and EDTA. When the organism was grown with cycloserine, lysis of the cell and in the presence of sucrose the conversion into spheroplasts were observed.
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