ペクチン酸およびアンモニウム塩を含むErwinia aroideaeの培養液における5-ホルミルピロール-2-カルボン酸の蓄積

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An unknown pyrrole was found in cultures of Erwinia aroideae grown with pectic acid and ammonium phosphate as substrates. It was also formed when pectic acid and ammo-nium phosphate were incubated with extracts of acetone-dried cells of this bacterium. With the extracts of the acetone-dried cells the optimum pH for the formation of the pyrrole was found to be 7.0 to 7.3. The addition of toluene enhanced the formation of the pyrrole and the shaking of the incubation mixture retarded it. The pyrrole was formed from pectic acid and 4, 5-unsaturated digalacturonic acid, but not from galacturonic acid and glucose. When amino acids were used instead of ammonium salts, the formation of the pyrrole was much delayed. The pyrrole was produced nonenzymatically from 4-deoxy-5-ketofructuronic acid, an intermediate in the metabolism of pectic acid by Erwinia aroideae, and ammonium phosphate. Further metabolism of this pyrrole was not observed with the washed cell suspension. The structure of pyrrole was found to be as 5-formylpyrrole-2-carboxylic acid.
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