微生物のクエン酸分解に關する研究 : (第1報) Bact. succinicumの有機酸代謝に就て

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1. The oxidation of acetate by pantothenic acid (PaA)-deficient Bact. succinicum was markedly increased by PaA-assimilation, while the effect of PaA assimilation on the anaerobic citrate decomposition was not recognized. Therefore, the decomposition of citrate seems too be catalyzed by another enzyme different from OCHOAs “condensing enzyme” 2. Malonate (1/200M) inhibited greatly the oxidation of acetate and pyruvate but not of succinate by washed cell suspension. This inhibition was overcome by the addition of small amounts of fumarate. Furtheremore, the succinate oxidation of the cell-free preparation was completely inhibited by malonate. Therefore, the observed inhibition for acetate and pyruvate oxidation isdue to inhibition for succinodehydrogenase. 3. The presence of tricarboxylic acid cycle was ascertained not only from the inhibition experiments with streptomycin, 2, 4-dinitrophenol, monofluoroacetate, arsenite and malonate, but also from the PaA-effect and the complete oxidation of acetate. 4. The inhibition experiment with α, α-bipyridyl and the metabolic behavior of PaA-deficient cells showed that anaerobic decomposition of citrate involved the different process from that in aerobic condition. 5. Dried preparation of cells was found to contain the enzyme which catalyzes the anaerobic decomposition of citrate.
社団法人日本農芸化学会の論文