微生物による酒石酸の分解について : (第2報)酒石酸分解の際のC14O2固定反応について

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Anaerobic decomposition of d-tartarate was conducted with the resting cells of Pseudo. monas iwognita strain 3l in the presence of radioactive bicarbonate. The succinic acid produced was isolated and degraded. The methylene carbons showed no radioactivity, while the carboxyl carbons the specific activity showed 2.5 times as high as final bicarbonate. Therefore this incorporation of C14 could not be explained by the mere exchange reaction between the succinic acid and CO2. Formic acid formed in a small amount was found to have about the same specific activity as that of final bicarbonate, while acetic acid showed no radioactivity. Residual tartaric acid was isolated and degraded. Carboxyl carbons showed weak activity but no activity was found in carbons of -CH(OH)-. (These results are presented in Table 1.) Dried preparation of cells was shown to have the strong activity of oxalacetic acid carboxylase. (Fig. 2) From these experimental results it was concluded that this decomposition may proceed va oxalacetate according to the Scheme II._??_ It is supposed that phosphorylated compounds or coenzyme-bound compouds are true intermediates, but these active forms were omitted for simplicity. C*=radioactive carbon.
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