通気培養におけるスパティフィラム小植物体の生育,見かけの光合成およびリブロ-ス-1,5-2リン酸カルボキシラ-ゼに及ぼす培地ショ糖濃度の影響について

概要

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Experiments were carried out to investigate an effect of sucrose concentration in the medium on growth, apparent photosynthesis, activity and content of ribulose-1, 5-bisphosphate carboxylase (RuBPCase) of plantlets cultured in vessels performed aeration. Growth of plantlet after 40 days was the best in 0% sucrose concentration, decreasing as sucrose concentration increased. Apparent photosynthetic rate decreased after 20, 30 and 40 days as sucrose was contained in the medium. In 2% sucrose concentration after 20 days and 3% sucrose concentration after 30 and 40 days, apparent photosynthesis was not found. The higher sucrose concentration in the medium lowered activity and content of RuBPCase in plantlet. plantlets which did not show apparent photosynthesis had also RuBPCase activity. Therefore, it seemed that decreasing sucrose concentration in the medium was effective for increasing of RuBPCase activity. The apparent photosynthetic rate was higher, as the result, the growth of plantlets may be promoted.
日本植物細胞分子生物学会の論文