Analysis of anticolon antibody and lymphocytophilic antibody in sera from patients with ulcerative colitis.

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The characteristics of anticolon antibody and lymphocytophilic antibody in sera from 41 patients with ulcerative colitis (UC) were examined by flow cytometry using a fluorescence activated cell sorter (FACS). The higher prevalence of both antibodies in the patients' sera was demonstrated than those previously reported. Anticolon antibody in sera was revealed to be IgG which had specificity for colon cells. This antibody bound to colon cell antigens in the form of monomer antibody via Fab' portion. These findings imply that anticolon antibody may participate in the mechanism of antibody dependent cell-mediated cytotoxicity (ADCC) in colon cell injury of UC patients. Lymphocytophilic antibody in UC sera was demonstrated to be mainly reactive with suppressor T cells, but also reactive with the other T cell subsets by two-color staining method. This antibody was not cytotoxic against peripheral T cells in the complement-dependent cytotoxicity assay. It was also shown that the antibody was heterogenous and might include anticolon antibody in part.All these results suggest that anticolon antibody and lymphocytophilic antibody may play an important role in the immunological pathogenesis of tissue injury in UC.
財団法人日本消化器病学会の論文