Counter Immunoelectrophoresis を応用した免疫学的便潜血反応に関する研究

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To develop an immunological test for fecal occult blood, we studied the specificities of rabbit antibodies against crude hemoglobin preparation by Drabkin's method, against purified HbAo preparation by Williams' method, and Hemoccult test.The antibody against crude hemoglobin (anti-cHb) reacted with erythrocytic non-hemoglobin proteins (NHP) as well as hemoglobin. The antibody against purified hemoglobin (anti-HbAo) did not react with NHP, bovine, chicken, nor with sheep blood in double immunodiffusion. To gain higher sensitivity to detect hemoglobin in stools, we employed counter immunoelectrophoresis (CIEP). Anti-HbAo also did not react with NHP, nor with these animal blood in this method.We examined the specificity of CIEP with anti-HbAo in stools from two groups. One is 11 patients with no gastrointestinal bleeding diagnosed by roentgenologic and endoscopic examinations (Group 1), and the other in 24 healthy asymptomatic adults younger than 30 years old (Group 2). Group 2 had 6 positives in Ouchterlony method with anti-cHb. By Hemoccult test, 1 and 2 positives in Group 1 and 2, respectively. By CIEP with anti-HbAo, there was no positive reaction in 2 groups. The positive reactions in Ouchterlony method with anti-cHb were shown to be derived from contaminating substances in stool, which were antigenically identical with NHP. This shows that specific antibody against purified hemoglobin must be used for immunological occult blood test.CIEP is rapid and easy to test, which is expected to be useful in screening for colorectal cancer.

Counter Immunoelectrophoresis を応用した免疫学的便潜血反応に関する研究

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