:Its Binding Power and Competition Among Cholephilic Dyes

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In order to clarify the role of ligandin, an intrahepatic carrier protein, in the hepatic clearance of cholephilic dyes, rat liver ligandin was conjugated to agarose beads. The binding power of the conjugated ligandin to bromsulfophthalein (BSP), indocyanine green (ICG) and bilirubin was compared and competitions of the dyes in binding were investigated.The following results were obtained: (1) the binding power of ligandin was about three times lesser than that of bovine albumin for BSP, (2) up to the concentrations tested, 10M per 1M ligandin for BSP and ICG and 2M per 1M ligandin for bilirubin, no competitions were seen among the dyes in binding to ligandin, and (3) the binding power of ligandin to the dyes were larger in the order of bilirubin, BSP and ICG, in the presence of equimolar amount of albumin in the media. It was concluded that ligandin was not the site of competition which are seen among the cholephilic dyes in the hepatic disposal.
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