脂質分解酵素の洗浄への応用に関する研究-5-トリオレインの加水分解率と洗浄性

概要

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In a series of studies on the application of lipases to detergency, the effects of the degree of hydrolysis of triolein soil by lipolytic enzymes, lipases, on removals were examined using triolein as a model triglyceride soil. First, the model mixed soil at various degrees of hydrolysis by <I>Candida cylindracea</I> lipase (Can) was washed by a several surfactant solutions. Secondly, as lipase detergency, a two step system of pre-soaking in lipase solution (Can and Mu) and washing by a surfactant solution, was carried out to avoid interactions between lipase and surfactants.<BR>In both cases, with increase in the degree of hydrolysis, removal of the triolein soil by anionic surfactants such as SDS, LAS and AOS, was highly improved due to interactions between hydrolysates and surfactants, while that by nonionic surfactants was not influenced by the degree of hydrolysis.<BR>However, a comparison of both cases, even at the same hydrolysis degree, indicated removal in lipase-detergent systems to surpass that in the model mixed soil. Considering the fact that hydrolysis reactions by lipase proceed at the oil/water interface, a removal can thus be explained as the efficient concentration of hydrolysates at oil/water interface. The application of lipases is concluded to be quite effective for the removal of triglyceride soil.
日本油化学会の論文