“大豆レシチン” 中のリン脂質諸成分の定量

概要

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Several methods for the quantitative analysis of phospholipids in commercial soybean lecithin ware compared. Analytical methods examined were as follows; Hanahan method based on silicic acid column chromatography, Hübscher-method which involves mild alkaline hydrolysis of total phospholipids and extraction of the resulting water-soluble esters and separation of the latter on ionexchange resins using sodium tetraborate-ammonium formate system for the elution, and Dawson-method based on the examination of phospholipid fractions by paper chromatography after mild alkaline hydrolysis. Among them, Hübschers-method was found, best in the determination of main phosphatide components.<BR>Quantitative determination of individual phospholipids by Hübscher-method in several lots of commercial soybean lecithin showed that there was little difference except the lot stoked over summer season in which the components of phosphatidylcholine and phosphatidylserine were less than others. In the lots of summer season stock, the package condition (can or paper bag) of soybean affected their phosphatide components. Can stock sample was less changed in the phosphatides and aceton insoluble lipids in the can stock soybean were more extracted than in the paper bag.
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