INHIBITORY ACTIVITY OF MITE IgG<SUB>4</SUB> ANTIBODY ON ANTIGEN-INDUCED HISTAMINE RELEASE FROM HUMAN PERIPHERAL BLOOD LEUKOCYTES
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Several reports have yielded conflicting results on the role of IgG<SUB>4</SUB> antibody on the surfaces of target cells in immediate type allergy. This study was performed to elucidate whether IgG<SUB>4</SUB> antibody inhibits IgE-mediated histamine release from target cells after antigenic stimulation, and whether it has reaginic activity.<BR>Serum was obtained from patients with nasal allergy receiving specific immunotherapy for housedust and mites. IgE and IgG<SUB>4</SUB> were enriched affinity chromatographically using monoclonal antibodies to IgE and IgG<SUB>4</SUB>, respectively, from the pooled sera. Both fraction revealed high antibody activity to Dermatophagoides Farinae antigen.<BR>Peripheral blood leukocytes from three non-allergic donors were passively sensitized with 100 or 300μgms of IgG<SUB>4</SUB> according to the method of Levy and Osler with a slight modification. Minimal or no histamine release was observed from leukocytes after challenge with both mite antigen and anti-IgG<SUB>4</SUB> monoclonal antibodies.<BR>Furthermore, to investigate the reaginic activity of IgG<SUB>4</SUB>, leukocytes from patients with nasal allergy were stimulated with anti-IgG<SUB>4</SUB> antibodies. The leukocytes of only three out of twenty patients released up to 10% histamine regardless of the IgG<SUB>4</SUB> concentration, while the other patients' leukocytes released minimal amounts of histamine.<BR>Two of the three above-mentioned non-allergic donors were passively sensitized with 100 or 300μgms of IgG<SUB>4</SUB> either one hour after sensitization with 100 ngs of IgE or simultaneously with the same amount of IgE. After sensitization with 100 ngs of IgE, one showed high-grade histamine release after challenge with 0.5μg/ml mite antigen and the other showed middle-grade release with 0.1μg/ml mite. With the presence of 300μgs of IgG<SUB>4</SUB>, histamine release was significantly inhibited in both donors regardless of the manner of sensitization. A control study was performed by absorbing IgG<SUB>4</SUB> with Sepharose 4B-mite complex. The inhibitory effect was minimized by the use of mite absorbed IgG<SUB>4</SUB>.<BR>In conclusion, these results strongly suggest that IgG<SUB>4</SUB> antibody plays an important role as a blocking antibody on the target cell surface in immediate type allergy.
- 社団法人 日本耳鼻咽喉科学会の論文
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