Characterization of Corticotropin Releasing Factor (CRF) and Arginine Vasopressin in Median Eminence Extracts on Sephadex Gel-filtration
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概要
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Hypothalamic median eminence extracts (MEE) were subjected to gel filtration on Sephadex G-25 and G-75 columns to characterize the corticotropin releasing factor (CRF) in relation to arginine vasopressin (AVP). CRF activity was measured using monolayer cultured anterior pituitary cells, and AVP was measured by radioimmunoassay.<BR>Sephadex G-25 chromatography of AVP immunoreactivity of MEE (NIAMDD-Rat HE-RP-1) showed three peaks on elution with 0.1 N HCl and four peaks on elution with 0.2 M acetic acid. But freshly prepared MEE showed only one peak on elution with 0.1 N HCl. These results suggest that AVP in NIAMDD-Rat HE-RP-1 is polymerized or aggregated on the Sephadex G-25 column, especially when eluted in 0.2 Macetic acid.<BR>Two main peaks of CRF activity appeared consistently on both Sephadex G-25 and G-75 chromatography. One was near the void volume and the other was retarded. The small molecular CRF (small-CRF) peak was coeluted with immunoreactive AVP and 1251-AVP, on both chromatographies on elution with 0.1 N HCl. The large molecular CRF (big-CRF) appeared between the void volume and 1-39 ACTH on Sephadex G-75 chromatography. Big-CRF from freshly prepared MEE had no AVP immunoreactivity. AVP showed CRF activity in pituitary cell cultures, but its CRF activity accounted for no more than 20% of the CRF activity of NIAMDD-Rat HE-RP-1. The log dose-response characteristics of the CRF activities of small-CRF and AVP differed.<BR>These results suggest that the median eminence has at least two substances with CRF activity: one is large molecular CRF, and the other is small molecular CRF which may have a vasopressin-like molecular weight. AVP may account for a part of the CRF activity of small molecular CRF but is not identical with genuine CRF.
- 社団法人 日本内分泌学会の論文
著者
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HASHIMOTO KOZO
Third Department of Internal Medicine, Okayama University Medical School
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OHNO NORIHITO
Third Department of Internal Medicine, Okayama University Medical School
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YUNOKI SHO
Third Department of Internal Medicine, Okayama University Medical School
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KAGEYAMA JINGO
Third Department of Internal Medicine, Okayama University Medical School
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AOKI YOSHIYUKI
Third Department of Internal Medicine, Okayama University Medical School
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TAKAHARA JIRO
Third Department of Internal Medicine, Okayama University Medical School
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OFUJI TADASHI
Third Department of Internal Medicine, Okayama University Medical School
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OHNO NORIHITO
Third Department of Internal Medicine Okayama University Medical School
関連論文
- Characterization of Corticotropin Releasing Factor (CRF) and Arginine Vasopressin in Median Eminence Extracts on Sephadex Gel-filtration
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