β-インドール酢酸のGordon-Weber法比色定量における亜硝酸の呈色妨害とその除去法

概要

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Nitrite may inhibit color development in Gorden-Weber's colorimetric determination of IAA, because it is likely decomposed by nitrite in an acidic solution under Salkowski reaction. Nitrite often occurs in plant tissues or microbial culture solution, hence the determination of IAA in such biological materials by Gordon-Weber's method is often restricted. Consequently, an appropriate procedure for eliminating the nitrite inhibition in Gordon-Weber's method is required. This paper has revealed the nature of the nitrite inhibition and presented a quite effective device which eliminates the effect of nitrite. It was shown by colorimetric analysis and paper chromatography that nitrite destroyed IAA rapidly, when the solution was acidic. When amines are added to a solution containing both IAA and nitrite, denitrification occurs, and it was most effectively done by sulfamic acid. An excess of this amine did not inhibit color development of IAA. Sulfamic acid was therefore recommended for eliminating the inhibitory effect of nitrite in determining IAA in biological materials which might contain nitrite by Gordon-Weber's method. Determination of IAA in Rhizobium culture solutions containing nitrite was conducted by Gordon-Weber's method with or without a pretreatment by sulfamic acid. The results showed that the pretreatment was quite satisfactory.
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