Improvement of a Venus-Based Bimolecular Fluorescence Complementation Assay to Visualize bFos-bJun Interaction in Living Cells
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概要
- 論文の詳細を見る
Bimolecular fluorescence complementation (BiFC) assay makes it possible to visualize protein-protein interactions in living cells. In this assay, Venus, a bright-yellow variant of green fluorescent protein, is known to produce fluorescent backgrounds due to non-specific interactions. In this study we found that the V150A mutation increased by 8.6-fold the signal-to-noise ratio in the BiFC assay of bFos-bJun interaction.
著者
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Nishimura Shigenori
Laboratory Of Biophysical Chemistry College Of Agriculture Osaka Prefecture University
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Sugimoto Kenji
Laboratory Of Applied Molecular Biology Department Of Applied Biochemistry Osaka Prefecture Universi
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NAKAGAWA Chika
Laboratory of Molecular Biology and Cell Informatics, Division of Bioscience and Informatics, Gradua
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INAHATA Kazuto
Laboratory of Molecular Biology and Cell Informatics, Division of Bioscience and Informatics, Gradua
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Inahata Kazuto
Laboratory Of Molecular Biology And Cell Informatics Division Of Bioscience And Informatics Graduate
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Nakagawa Chika
Laboratory Of Molecular Biology And Cell Informatics Division Of Bioscience And Informatics Graduate
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