養殖ウナギの赤点病に関する研究-5-原因菌に対するニホンウナギの免疫応答〔英文〕

概要

論文の詳細を見る
Immune response of the Japanese eel (Anguilla japonica; average weight: 70g) to formalinkilled Pseudomonas anguilliseptica cells administered intramuscularly was studied. Experimental fish had no specific agglutinin acquired naturally. Agglutinating antibody was detected 2 weeks after vaccination at water temperatures from 15°C to 28°C and the antibody response persisted over a period of 7 weeks (maximum titer: 256), and even at 10°C it was detected as late as 7 weeks after vaccination (titer: 2). And the antibody response in the eels immunized at about 20°C was maintained over 5 months through the low temperature period (minimum: 7°C). The use of Freund's complete adjuvant had a promotive effect on the development of agglutinin titer (maximum: 4096). Vaccinated eels were highly protected against experimental infection with virulent P. anguilliseptica, and this protective immune response was demonstrated even after wintering. These results suggest that this vaccination method will provide cultured eels with protective immunity against naturally occurring red spot disease.
日本水産學會の論文