薄層クロマトグラフィ-による魚介類中のホルムアルデヒドの検出

概要

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A convenient method, based on thin-layer chromatographic procedures, was developed to identify formaldehyde which circumstantially occurs in aquatic animals. In the experiments described, the formaldehyde in muscle was extracted with petroleum ether after converting it into 2, 4-dinitrophenylhydrazone. The extract was then evaporated to dryness and the residue was re-extracted with a small volume of toluene. The final extract was then applied on activated silica gel G plates, and developed with a solvent consisting of hexane-ethanol-acetic acid (90:10:3). The confirmatory evidence of formaldehyde in muscle was obtained from parallel experiments conducted with authentic 2, 4-dinitrophenyl-hydrazones of C1- and C2-alkanals. The presence of formaldehyde in muscle of fish and other aquatic animals was successfully confirmed by the adapted method, and it was found that the aldehyde exists in all of the muscles so far as examided except in the cyprinid, Carassius auratus.
公益社団法人日本水産学会の論文