魚体表面の粘性物質に関する研究-V : ドジョウの体表面粘性物質構成糖の再検討

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The component sugars of the mucus-polysaccharide from the external mucous substance of loach was reported in the previous paper1): mucus-polysaccharide acid hydrolyzate was separated into three fractions based on Rf values by paper chromatography, and the component sugars was identified. In the present paper, the hydrolyzate was divided into neutral, basic and acidic fractions by using ion-exchange resins (Fig. 1), and the component sugars in each fraction were analyzed qualitatively. The results obtained are as follows: 1) In the neutral fraction, ribose, galactose and glucose were identified. Although the presence of arabinose was reported in the previous paper1), this sugar could not be detected clearly (Fig. 2). 2) In the basic fraction, glucosamine and galactosamine were identified indirectly as arabinose and lyxose, respectively, after degradation of the fraction with ninhydrin. 3) In the acidic fraction, a hexuronic acid was detected by the WILLIAMSs method (Fig. 3). The acid seemed to be glucuronic acid, as judged from the DISCHEs reaction (Fig. 4).