魚体表面の粘性物質に関する研究-I : ドジョウの体表面粘性物質の化学的性質

概要

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The present work is an attempt to elucidate the chemical properties of the external mucous substance of loach. The precipitation test, the identification of amino acids and carbo-hydrates by paper chromatography, and the quantitative analysis of the polysaccharide were employed. The external mucous substance of loach was prepared by dipping living loaches in ethanol, scraping the mucous substance carefully from the whole surface of the body, washing it with ethanol and drying it in vacuo. Greyish powder was obtained. Sample for identification of amino acid component was repeatedly dissolved in 0.1% sodium hydroxide and precipitated at pH 4.0 with dilute hydrochloric acid. Sample for analysis of carbohydrate was prepared by hydrolyzing with pepsin and trypsin. The results obtained are as follows: 1) The result of precipitation test of the external mucous substance is shown in Table 1. All reagents used for the protein precipitation took effect, but heating was futile. 2) 16 amino acids were identified and the presence of an unidentified amino acid was detected (Fig. 1). 3) Ribose, arabinose, glucose, galactose, glucosamine and chondrosamine were identified. 4) It seems that the polysaccharide of the external mucous substance is composed of hexose and hexosamine at equimolar ratio.