防腐劑の作用機構に関する研究-III : Cysteine代謝に及ぼすFormaldehydeの影響

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概要

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• It has been found that the bacteriostatic action of formaldehyde is reversed by cysteine and that the reversal is attributed to the formation of thiazolidine-4-carboxylic acid (TCA) due to the reaction between the two substances.* In this paper, some attempts have been made to ascertain whether formaldehyde inhibits the cysteine metabolism of bacteria or not. The results obtained are as follows. (1) In a synthetic medium TCA, at its concentrations over 7. 5×10-4 M, exerts its inhibitory action on the growth of E. coli as reported by BEERSTECHER10) (Table 1), while in a broth medium little effect of TCA is observed even at its far higher molar concentrations than that of formaldehyde used for the experiments of bacteriostasis* (Table 2). The laatter fact may demonstrate that the bacteriostatic action of formaldehyde is not due to the effect of TCA. (2) At the concentrations below 11×10-4 M, TCA, plays the role of sulfur source to the growth of E. coli in a synthetic medium free from sulfur compound (Table 3) and its efficacy as sulfur source is nearly as same as sodium sulfate, cysteine and others (Table 4). From the above results, it may be said that formaldehyde dose not inhibit the cysteine metabolism of bacteria owing to the formation of TCA. (3) It has been further proved that the cells of E. coil dehydrogenate TCA (Table 5) and that a certain enzyme participates in this dehydrogenation (Table 6, Figs. land 2).

• 公益社団法人 日本水産学会の論文

著者

• 山田 金次郎

  東海区水産研究所

• 有賀 佐代子

  東海区水産研究所

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