Water-soluble Vitamin Requirements of Carp-II:Requirements for p-Aminobenzoic Acid and Inositol

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1. p-Aminobenzoic acid and inositol were eliminated individually from the basal diet used by HALVER et al., and the requirements of young carp for the vitamins were studied. 2. In feeding trials for 16 weeks, no deficiency symptom of p-aminobenzoic acid was observed. Moreover, addition of sulfanilamide to the diet revealed no effect on growth and numbers of erythrocytes. From these results p-aminobenzoic acid is concluded to be nonessential for young carp under the experimental conditions. 3. The deficiency symptoms of inositol were characterized by skin lesions, loss of appetite and retarded growth. The younger carp indicated the deficiency symptoms at a higher rate than the elder ones. There were some distinct indications of supply of the vitamin by biosynthesis. 4. Under the experimental conditions adopted, the requirement for inositol was found to be 7 ?? 10mg per kg of body weight per day (44mg per 100g of diet) on the basis of body weight gain.
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