Studies on platelet surface properties in patients with hepatic cirrhosis by isoelectric equilibrium analysis.

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A characterization of the platelets treated with sodium citrate has been made by isoelectric equilibrium analysis. Isoelectric focusing was carried out in a 50ml column fitted with platinum electrodes. An aliquot of 1-5×10<SUP>8</SUP> platelets were focused at 0-1°C for 4 hours by post-pH equilibrium loading of the platelets in a sucrose linear gradients (0-50%) containing 1% ampholine at pH 3-10. After focusing, the column was drained. The pH and the OD at 420nm were determined in 1.0ml fraction.<BR>Unmodified platelets of healthy subjects show a surface isoelectric point (pI) of 4.22. Those of hepatic cirrhosis show pI between 3.80 and 4.50. When surface amino groups are blocked by formaldehyde and dimethyl sulfoxide, the platelets of healthy subjects have pI of 3.92 and 4.04, respectively. Platelets treated with neuraminidase are isoelectric at pH 4.54.
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