Investigation of sulfate group position of urinary sulfated ursodeoxycholic acid in patients during ursodeoxycholic acid administration

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The position of sulfate group of sulfated ursodeoxycholic acid in urine was investigated, using bile acid sulfate sulfatase (BSS) which could specifically desulfate on the 3 position of sulfate group of sulfated bile acid, column chromatography and gas liquid chromatography method. Ursodeoxycholic acid 3-monosulfate was desulfated and transformed into 3β, 7β-dihydroxy-5β-cholanoic acid by BSS. Concentration of urinary sulfated ursodeoxycholic acid in seven patients with chronic liver disease (two patients with primary biliary cirrhosis, two chronic active hepatitis and three compensatory liver cirrhosis) during oral administraion of 600mg/day ursodeoxycholic acid was 3.88±1.7μg/ml (mean±SE). Ursodeoxycholic acid 3-monosulfate was dominant (97.3%) in composition of sulfated ursodeoxycholic acid, and the other monosulfate was only 2.7%. Ursodeoxycholic acid disulfate was not detected in our patients.
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