L929線維芽細胞の増殖性と接着性に及ぼす基材表面の微細凹凸形状の影響

概要

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In this study, the effects of Fine Particle Peening (FPP) treatment on adhesion and proliferation in L929 fibroblast were investigated. FPP treatments were performed on the Ti-6Al-4V alloy specimen by using TiO2 (T series) , glass beads (G series) and Alumina (Al series) shot particle. The polished specimen (P series) was also prepared. Cell culture tests on the specimen were carried out with L929 fibroblast after 6 hours, 1, 3 and 5 days culture. Cell proliferation was evaluated by counting the number of cells by MTT assay. Cell adhesion strength was also measured by counting the number of cells detached by fluid-induced shear stress. The FPP treated surface by using shot particle of varied materials, which have different surface character. The TiO2 series showed good cell proliferation. This was because the protein adsorption of TiO2 series encourage cell adhesion. The detachment cells on the specimen were observed after share stress loading. The FPP treated series showed good cell adhesion. This was because the microscopically surface topography encourage cell adhesion.