FLUOROMETRIC ASSAY OF TISSUE HISTAMINE
スポンサーリンク
概要
- 論文の詳細を見る
There have been numerous studies on the distribution of endogenous histamine (1-5). Many mammalian tissues contain large to small amounts of histamine preformed. Amongst them skin, stomach and lung contain extremely high concentration of histamine. These organs are exposed to the outside directly or indirectly and prone to traumatic injury. The tissue level of histamine has been estimated with the bioassay procedure using isolated guinea-pig intestine or uterus. The fluorometric analysis of tissue histamine was devised by Shore <I>et al</I>. (6). The method involves extraction of histamine to n-butanol from alkalinized perchloric acid tissue extracts, return of the histamine to an aqueous solution and condensation with o-phthalaldehyde (OPT) to yield a product with strong and stable fluorescence. This fluorometric determination of tissue histamine is simple, precise and sensitive, but inadequate for the analysis of brain histamine because of the presence of interfering substance. Kremzner and Pfeiffer (7) have shown that the major interfering substance, spermidine, is separable from histamine by the use of a phosphorylated cellulose column. After that, Medina and Shore (8) modified the Kremzner and Pfeiffer procedure so as to increase the sensitivity. In the present experiments the Medina and Shores modification for the fluorometric analysis of tissue histamine was carefully re-examined for the application to pharmacological studies.
- 社団法人 日本薬理学会の論文
著者
-
藤原 元始
Department of Pharmacology, Faculty of Medicine, Kyoto University
-
倉橋 和義
Department of Pharmacology, Faculty of Medicine, Kyoto University
関連論文
- RESPONSE TO SYMPATHETIC NERVE STIMULATION AND NORADRENALINE IN ATRIA TREATED WITH RESERPINE AND IN ATRIA FROM RABBITS PRETREATED WITH RESERPINE
- EFFECTS OF N, N-DIPHENYLDICARBAMATE-1, 1-DEMETHYLOL CYCLOPENTANE (CAMALON) ON THE SPINAL CORD REFLEXES
- FLUOROMETRIC ASSAY OF TISSUE HISTAMINE
- PHARMACOLOGICAL ACTION OF OCTOPAMINE WITH SPECIAL REFERENCE TO BIOCHEMICAL CONVERSION TO NORADRENALINE
- INHIBITION OF CATECHOL-O-METHYLTRANSFERASE BY HYDROXYBENZENES AND RELATED COMPOUNDS
- THE UPTAKE OF DOPAMINE-14C AND ITS CONVERSION TO NORADRENALINE-14C IN RATS
- THE EFFECTS OF RESERPINE ON THE SPONTANEOUS CONTRACTION OF THE ISOLATED ATRIUM OF RABBIT TREATED WITH MONOAMINE OXIDASE INHIBITORS
- FLUORESCENCE HISTOCHEMICAL FINDINGS OF THE STOMACH WALLS IN RESPONSE TO ULCEROGENIC STIMULI IN RATS
- FLUORESCENCE HISTOCHEMICAL DEMONSTRATION OF ADRENERGIC NERVE FIBERS IN THE VAGUS NERVE OF CATS AND DOGS
- HISTOCHEMICAL DEMONSTRATION OF NORADRENALINE IN RAT SALIVARY GLANDS
- EFFECTS OF THE ADRENERGIC DRUGS ON THE CHRONO- AND INOTROPIC RESPONSES TO THE CHOLINERGIC STIMULATION OF THE ISOLATED GUINEA-PIG'S ATRIA
- HISTOCHEMICAL DEMONSTRATION OF MONOAMINES IN THE THYMUS OF RATS
- EFFECTS OF VAGAL STIMULATION ON THE TRANSMEMBRANE ACTION POTENTIALS OF ATRIUM ISOLATED FROM GUINEA PIG
- MODIFICATIONS OF THE VAGAL EFFECT ON THE HEART AND THE CARDIAC METABOLISM BY RESERPINE IN VITRO
- EFFECTS OF TEMPERATURE CHANGE, OXYGEN DEPRIVATION AND CATIONS ON THE ATRIAL RESPONSES TO VAGAL STIMULATION
- EFFECTS OF 10-METHOXYDESERPIDINE AND RESERPINE ON THE SKIN AND RECTAL TEMPERATURE IN RABBITS
- DEPLETION OF ATRIAL NOREPINEPHRINE INDUCED BY RESERPINE IN VITRO
- TISSUE DISTRIBUTION OF EXOGENOUSLY ADMINISTERED, AND ENDOGENOUSLY ACCUMULATED OCTOPAMINE IN RATS
- EFFECTS OF 10-METHOXYDESERPIDINE ON THE ECG CHANGES INDUCED BY VASOPRESSIN IN UNANESTHETIZED RABBIT
- THE EFFECT OF RESERPINE ON PHOSPHORYLASE ACTIVITY IN THE ISOLATED RABBIT ATRIA