BIOTRANSFORMATION OF COUMARIN DERIVATIVES (2) OXIDATIVE METABOLISM OF 7-ALKOXYCOUMARIN BY MICROSOMAL ENZYMES AND A SIMPLE ASSAY PROCEDURE FOR 7-ALKOXYCOUMARIN O-DEALKYLASE
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概要
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The in vitro biotransformation of 7-alkoxycoumarin by rat liver microsomes was studied to develop a simple and accurate assay procedure for 7-alkoxycoumarin O-dealkylase. 7-Alkoxycoumarin was converted to the O-dealkylated metabolite, 7-hydroxycoumarin, by aerobic incubation of the parent compound with microsomes and NADPH, but the decreased amount of 7-alkoxycoumarin in the reaction mixture was several times higher than that of the 7-hydroxycoumarin produced during the incubation. The thin-layer chromatogram of the ether extractable metabolites in the reaction mixture showed the existence of several fluorescent metabolites including 7-hydroxycoumarin. Fluorescent properties of the parent compound, 7-alkoxycoumarin, and most of the metabolites differed from that of 7-hydroxycoumarin, but the reaction cofactor, NADPH, showed similar properties. Treatment of the reaction mixture with perchloric acid resulted in conversion of NADPH to the non-fluorescent form without any effect upon the fluorescent properties of 7-hydroxycoumarin and its related compounds. Based on these properties, an improved and simple in vitro fluorometric assay of the O-dealkylation of 7-alkoxycoumarin was developed. The method is applicable to routine determination of O-dealkylase activity in both isolated microsomes and whole homogenate. Species differences in the substrate specificity of the O-dealkylation reaction and in the responsiveness of animals to the inducer were observed even with use of the liver homogenate obtained from untreated and phenobarbital- or β-naphthoflavone-pretreated animals, similar to what was observed with the microsomal system.
- 社団法人 日本薬理学会の論文
著者
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Matsubara Takashi
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Touchi Akira
Shionogi & Co. Ltd. Developmental Research Laboratories
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OTSUBO Shigemi
Shionogi Research Laboratories, Shionogi & Co., Ltd.
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YOSHIHARA Emiko
Shionogi Research Laboratories, Shionogi & Co., Ltd.
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Matsubara Takashi
Shionogi Research Laboratories
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Yoshihara Emiko
Shionogi Research Laboratories
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MATSUBARA TAKASHI
Shionogi Research Laboratories, Shionogi & Co., Ltd.
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OTSUBO Shigemi
Shionogi Research Laboratories, Shionogi & Co., Ltd.
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TOUCHI AKIRA
Shionogi Research Laboratories, Shionogi & Co., Ltd.
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YOSHIHARA Emiko
Shionogi Research Laboratories, Shionogi & Co., Ltd.
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