モルモツト内耳の酸性ムコ多糖に関する生化学的および組織化学的研究
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The important role of acid mucopolysaccarides (AMP) in the cochlea has been already pointed out by Hayashido, Bleanger, Dohlman and Müsebeck. In Hayashidos report chondroitin sulfate (ChS) was supposed as the most important AMP to keep the cochlear function.The author have studied on the enzymatic synthesis of ChS in the guinea pig cochlea.Five guinea pigs were guillotined and their cochleae were homogenized with 0.25 M sucrose.The homogenate was centrifugated at 18, 000xg for 10min, and the supernatant fluid was collected.All procedures were done at 4°C. Using the crude extract of cochleae prepared previously according to above method, PAPS (3-phosphoadenosine-5-phosphosulf ate) -synthesizing enzyme system and ChS-transferase were evaluated by means of electrophoresis (citrate buffer, pH5.9, 16 V/cm, 3h) and radioautography.In acoustic trauma, the PAPS-synthesizing enzyme system decreased at first, and then increased. In KM intoxication, the PAPS enzyme activity decreased. On the other hand, ChS-transferase in both condition showed its almost normal activity. These observation would suggest that decrease of the enzyme activity of PAPS system may be one of the causes for hard of hearing.Histochemical investions for KM intoxicated cochlea were also performed by several staining methods. The decreased reaction of the hyaluronidase resistant sulfate AMP, probably ChS in the tectorial membrane and hyaluronic acid in the limbus was investigated.The perceptive mechanism of sound vibration in the cochlea may be realized through the knowledge of AMP obtained in this paper, included the action as anion, and the clinical use of the adenosine-5-phosphosulf ate or PAPS for perceptive hearing loss may be suggested according to the above results.
- 社団法人 日本耳鼻咽喉科学会の論文
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