Experimental chemotherapy using a model for liver metastasis of colon cancer (The effect of mitomycin administrated into the portal vein).:(The Effect of Mitomycin Administrated into the Portal Vein)

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A mouse model has been developed for studying the treatment of colorectal liver metastasis. Single cell suspensions were obtained from transplantable mouse colon cancer (colon 26, BALB/c). Tumor cells (103, 104, 105) were inoculated into the portal vein of syngenic mice. On the day 8 after inoculation, all mice showed microscopic liver metastasis. No macroscopic metastsis was observed. On the day 18 after inoculation of 105 cells, all mice showed macroscopic liver metastasis. This tumor also proved to be sensitive to mitomycin (MMC). This model seemed to be useful for studying the treatment of colorectal liver metastsis. <BR>This model was applied to experimental chemotherapy. We investigated the inhibitory effect of intraportal MMC administration on the growth of hepatic micrometastasis. On the day 8 after 105 tumor cells inoculation, one group of mouse were relaparotomized and MMC (70μg/body) was administered into the portal vein (treated group). Another group of mouse were observed without treatment (control group). When mice were killed 18 days after inoculation, the average number of metastatic nodules was smaller, and the mean liver weight was lower in the treated group. In addition, significant prolongation of survival days was achieved in the treated group. These results indicated that intraportal administration of sensitive drugs might inhibit the growth of micrometastasis of the liver.
日本大腸肛門病学会の論文