2細胞期胚の割球融合により作出したマウス4倍体胚の生存性

概要

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The developmental ability of tetraploid mouse embryos (4 N embryos) produced by electrofusion at the 2-cell stage was examined in vitro and in vivo. The effect of aggregating two 4 N embryos at the 2-cell stage was also examined. The results obtained were as follows.Tetraploid embryos developed to blastocysts at approximately the same rate as in control embryos (76 vs 65%), but they had reduced cell numbers (30±5 vs 64±11). On 11.5 days post coitum after transfer to recipients, 79% of 4 N embryos implanted but none of them had developed to fetuses. Although aggregated 4 N embryos formed blastocysts at a high rate (85%), fetuses were not observed even on 9.5 days post coitum. These results suggest that the poor developmental ability of 4 N embryos was not caused by reduced cell numbers.
社団法人日本畜産学会の論文