Androglnの定量に於ける幼雛鶏冠反応法に加えた2-3の改良点に就て
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概要
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1) Some improvements were made to the chick's comb test by innunction method for assay of androgens.<BR>2) Sesame oil, ethyl alcohol and propylene glycol were innuncted on the skin of the back of a human hand. Then time was measured until the fluids were absorbed by the skin, and the degree of diffusion of the fluids on the skin recorded of the three fluids, alcohol diffused to the largest extent, and the absorption of sesame oil was three times longer than that of, propylene glycol. So propyleneglycol was used in this experiment.<BR>3) It seems desirable to apply a method using comb weight with covariance for body weight, as the criterion of response.<BR>4) Administration of hormone carried out twice a day seemed to induce the weakness of chickens and increase the variance of response rather than increase the degree of response.<BR>5) According to many authors, a dose of the applied fluid was 0.05cc but in this experiment even a dose of 0.01cc was too large, and the degree of response and the slope of the dose-response line were greatly increased by the administration of 0.005cc.<BR>6) In the 4-point assay method, only 28 experimental chickens are enough to obtain significant results if the fiduciary limit is established to ±10 per cent of mean (P=0.95). If 100 chickens are used, such limit can be established to ±5 per cent.<BR>7) Better results might be expected if a dose smaller than that used in this experiment was applied. As loss of the fluid by diffusing and touching is neglected when such a smaller dose is applied, it may be more desirable to use oil than propylene glycol.
- 社団法人 日本畜産学会の論文
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