マウスの(腦)下垂体除去法

概要

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Up to this time hypophysectomy in the mouse seems to have been considered as almost impracticable in our country, but the increasing demand for the experimental use of hypophysectomized mice in studies of pituitary gland and its relations to other endocrine glands has made it desirable to develop for these animals a reliable, easy and rapid operative technic.The only descriptions of the operative technics on mice that I could find are discussed in only two papers, one by Mr. SELYE et al. (34) and one by Mr. THOMAS (38), and both of these seem to be somewhat cumbersome and difficult for routine practical use.Therfore, the procedures of these earlier workers on both the rat and the mouse were tried and certain features of their methods adopted and some new instruments used, so that the operation may accommodate itself more easily to routine practical use.The procedures and instruments which serve best in our hand after much experience are here described and illustrated in detail.The technic described here presents the advantages of simplicity, speed, total exposure of the pituitary and its removal under direct vision, the advantages of an operation easily performed both at an earlier age (weighing as little as 6 gram.) and at an older one (weighing over 20 gram.) with low mortality.
社団法人日本畜産学会の論文