高分圧の炭酸ガスによるヤギ精子の好気的グルコ-ス代謝の抑制

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Effect of high levels of CO2 on the aerobic metabolism of glucose by goat spermatozoa was investigated in Ca-free Krebs-Ringer phosphate solution of a constant pH of 7.0. Spermatozoa were washed twice and incubated with glucose or glucose-U-14C at 37°C under the atmosphere of different levels of CO2 in air, oxygen or nitrogen. Results obtained are as follows. 1) Aerobic glycolysis of spermatozoa was depressed as the CO2 level increased, and the degree of its inhibition appeared to be almost the same as observed under anaerobic conditions. The inhibitory effect of higher levels of CO2 was remarkable on lactate production, thus the ratio of lactate production to glucose utilization decreased as the CO2 level increased. However, an appreciable accumulation of organic acids other than lactate did not occur in the reaction mixture incubated under 75% CO2 in air. 2) Production of 14CO2 was also depressed as the CO2 level increased. Production of 14CO2 under 75% CO2 in air was greater than that under 75%CO2 in nitrogen but it was significantly depressed and lactate production was stimulated when spermatozoa were incubated with fluoroacetate (1.0 mM) or potassium cyanide (0.1 and 1.0 mM). These results suggest that the respiration of spermatozoa might be greatly, but not completely inhibited under high levels of CO2.
社団法人日本畜産学会の論文