生体試料を対象とした電子顕微鏡断層像の三次元可視化

概要

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In this paper, we propose a visualization method for electron microscope tomogram intended of biological sample. As for electron microscope tomogram, the application to diagnosis is expected. In order to observe the 3D structure from electron microscope tomogram, most important process is extraction of contours from those 2D images. However, it is difficult to extract the contours automatically because of their typical noise and artifacts. The proposed method automatically extracts the contours by using three-dimensional level set method. In general, the result of the extraction with level set method depends on the initial contours and its parameters. These parameters are usually set manually. Our method automatically generates the initial contours and decides the fittest parameters of level set method. We verify the effectiveness of our method with the experiment to compare the result of the proposed method with manual extraction.